FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
flw, trx
FlyBase ID
FBig0000096958
Interaction Network
Interactions Browser links
flw network
trx network
Reported Interactions
FBrf0155742-2.Y2H
Reference
Rudenko et al., 2003
Description
physical association
Assay
Collection
Source/Stage
organism / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
flw 

Pp1β9C

bait

fused to Gal4 DNA-binding domain

trx 

trx

prey

fused to GAL4 activation domain

trx 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
trx 
PP1 interaction domain
sufficient binding region
aa 379-1030aa 302-479

coordinates relative to trx-PA

coordinates relative to trx-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from third instar larval cDNA expression library).

(Rudenko et al., 2003)

Two-hybrid system: yeast Gal4-BD/GAL4-AD

(Rudenko et al., 2003)
FBrf0155742-4.Y2H
Reference
Rudenko et al., 2003
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
flw 

Pp1β9C

bait

fused to Gal4 DNA-binding domain

trx 

trx

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast Gal4-BD/GAL4-AD

(Rudenko et al., 2003)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Rudenko et al., 2003)
FBrf0180133-1.PD.WB
Reference
Rudenko et al., 2004
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
trx 

TRX

bait

GST tag

trx 
trx 
flw 

PP1β9C

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
trx 
interaction domain
sufficient binding region
aa 302-479

coordinates relative to trx-PA

trx 
interaction domain
mutation disrupting interaction
F435A

coordinates relative to trx-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transgenic construct.

(Rudenko et al., 2004)
FBrf0180133-2.coIP.WB
Reference
Rudenko et al., 2004
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
trx 

trx

bait
flw 

PP1β9C

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extracts of transgenic fly line; bait produced from endogenous gene; prey derived from transgenic construct

(Rudenko et al., 2004)
FBrf0194330-7.Y2H
Reference
Bennett et al., 2006
Description
physical association
Assay
Collection
Source/Stage
organism / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
flw 

PP1β9C

bait

fused to GAL4 DNA-binding domain

trx 

trithorax

prey

fused to VP16 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from third instar larva cDNA expression library).

(Bennett et al., 2006)

Two-hybrid system: yeast GAL4-BD/VP16-AD

(Bennett et al., 2006)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)