FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Rgl, Ras85D
FlyBase ID
FBig0000096966
Interaction Network
Interactions Browser links
Rgl network
Ras85D network
Reported Interactions
FBrf0155960-1.Y2H
Reference
Mirey et al., 2003
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Ras85D 

Ras1

bait
Rgl 

Rgl

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

(Mirey et al., 2003)

Two-hybrid system: yeast LexA-BD/GAL4-AD

(Mirey et al., 2003)
FBrf0155960-2.Y2H
Reference
Mirey et al., 2003
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rgl 

Rgl

bait
Rgl 
Ras85D 

Ras1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Rgl 
C-terminal region
sufficient binding region
aa 488-774
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast LexA-BD/GAL4-AD

(Mirey et al., 2003)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

(Mirey et al., 2003)

Rgl binds activated G12V mutants of Ras85D, but not dominant negative S17N mutants.

(Mirey et al., 2003)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)