FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Zasp52, Zasp66
FlyBase ID
FBig0000097000
Interaction Network
Interactions Browser links
Zasp52 network
Zasp66 network
Reported Interactions
FBrf0221051-3.coIP.WB
Reference
Katzemich et al., 2013
Description
physical association
Assay
Collection
Source/Stage
adult thorax / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Zasp66 

Zasp66

bait

GFP tag

Zasp52 

Zasp52

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult thorax of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Katzemich et al., 2013)
FBrf0244303-2.Y2H
Reference
González-Morales et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Zasp66 

Zasp66

bait

fused to GAL4 DNA-binding domain

Zasp52 

Zasp52

prey

fused to GAL4 activation domain

Zasp52 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Zasp52 
LIM2 or LIM3 domains
sufficient binding region
unspecified
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD.

(González-Morales et al., 2019)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(González-Morales et al., 2019)
FBrf0244303-4.Y2H
Reference
González-Morales et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Zasp52 

Zasp52

bait

fused to GAL4 DNA-binding domain

Zasp52 
Zasp66 

Zasp66

prey

fused to GAL4 activation domain

Zasp66 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Zasp52 
LIM2 or LIM3 domains
sufficient binding region
unspecified
Zasp66 
ZM domain
necessary binding region
aa 257-282
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(González-Morales et al., 2019)

Two-hybrid system: yeast GAL4-BD/GAL4-AD.

(González-Morales et al., 2019)
FBrf0244303-8.BiFC.FM
Reference
González-Morales et al., 2019
Description
physical association
Assay
Collection
Source/Stage
adult somatic muscle cell of thorax / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Zasp66 

Zasp66

bait

YFP tag, N-terminal fragment

Zasp52 

Zasp52

prey

YFP tag, C-terminal fragment

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult thoracic muscle of transgenic fly line; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.

(González-Morales et al., 2019)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)