Interaction in vitro; bait derived from wild-type ovary extract; prey produced and labeled by in vitro transcription.
positive control
Interaction in vitro; bait produced and labeled by in vitro transcription; prey derived from wild-type adult female ovary extract.
Source was adult female ovaries; bait produced and labeled by in vitro transcription; prey produced as transgenic fusion protein in bacteriophage (prey from adult ovary cDNA expression library).
Interaction in vitro; bait produced and labeled by in vitro transcription; prey produced as a recombinant fusion protein in bacterial system.
Source was adult ovary of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.
Interaction in vitro; bait produced by in vitro transcription; prey derived from wild-type ovary extract.
GRNA chromatography: grk RNA containing a BoxB sequence of the λ phage was bound to a fusion of GST with the 21 residue N antiterminator peptide.
The grk-GST particles were pre-bound to resin and incubated with ovary extract.
Source was adult female ovary of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.
Positive control.
Source was adult ovaries; bait produced from endogenous gene; prey produced from endogenous gene.
Interaction increased in spn-B mutant.
