FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
flw, Sara
FlyBase ID
FBig0000097053
Interaction Network
Interactions Browser links
flw network
Sara network
Reported Interactions
FBrf0151442-1.Y2H
Reference
Bennett and Alphey, 2002
Description
physical association
Assay
Collection
Source/Stage
organism / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
flw 

PP1β9C

bait

fused to GAL4 DNA-binding domain

Sara 

Sara

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from larval cDNA expression library).

(Bennett and Alphey, 2002)

7 independent clones of Sara were isolated in this two-hybrid screen.

(Bennett and Alphey, 2002)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Bennett and Alphey, 2002)
FBrf0151442-5.coIP.WB
Reference
Bennett and Alphey, 2002
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Sara 

Sara

bait

Myc tag

Sara 
flw 

PP1

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Sara 
PP1 binding motif
mutation disrupting interaction
aa 678

F678A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of COS-7 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Bennett and Alphey, 2002)
FBrf0151442-6.coIP.WB
Reference
Bennett and Alphey, 2002
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
flw 

PP1

bait

HA tag

Sara 

Sara

prey

Myc tag

Sara 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Sara 
PP1 binding motif
mutation disrupting interaction
aa 678

F678A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of COS-7 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Bennett and Alphey, 2002)
FBrf0194330-10.Y2H
Reference
Bennett et al., 2006
Description
physical association
Assay
Collection
Source/Stage
organism / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
flw 

PP1β9C

bait

fused to GAL4 DNA-binding domain

Sara 

Sara

prey

fused to VP16 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from third instar larva cDNA expression library).

(Bennett et al., 2006)

Two-hybrid system: yeast GAL4-BD/VP16-AD

(Bennett et al., 2006)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)