FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
msl-1
FlyBase ID
FBig0000097097
Interaction Network
Interactions Browser links
msl-1 network
Reported Interactions
FBrf0191197-1.coIP.WB
Reference
Li et al., 2005
Description
physical association
Assay
Collection
Source/Stage
organism / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
msl-1 

MSL1NHA

bait

HA tag

msl-1 

MSL1

prey
msl-1 
msl-1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
msl-1 
interaction domain
sufficient binding region
aa 27-265

coordinates relative to msl-1-PA.

msl-1 
interaction domain
necessary binding region
aa 27-50

coordinates relative to msl-1-PA.

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adults of transgenic fly line; bait produced from tagged transgenic construct; prey produced from transgenic construct.

(Li et al., 2005)
FBrf0220106-7.coIP.WB
Reference
Hallacli et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
msl-1 

Msl1

bait

FLAG tag

msl-1 

Msl1

prey

Myc tag

msl-1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
msl-1 
coiled coil
mutation disrupting interaction
aa 114-145

V114D, M121D, M142D, V145D quadruple mutation

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of SL2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Hallacli et al., 2012)

Dimerization of msl-1 is not affected by mutations that disrupt the binding of msl-1 to msl-2, msl-3 or mof.

(Hallacli et al., 2012)

Mutated versions of FLAG-tagged msl-1 were tested for their ability to dimerize with Myc-tagged wild-type msl-1.

(Hallacli et al., 2012)
FBrf0220106-8.coIP.WB
Reference
Hallacli et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
msl-1 

Msl1

bait

FLAG tag

msl-1 

Msl1

prey

Myc tag

msl-1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
msl-1 
coiled coil
mutation disrupting interaction
aa 114-145

V114D, M121D, M142D, V145D quadruple mutation

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of Kc cell line; bait produced from transfected construct; prey produced from transfected construct.

(Hallacli et al., 2012)

Dimerization of msl-1 is not affected by mutations that disrupt the binding of msl-1 to msl-2, msl-3 or mof.

(Hallacli et al., 2012)

Mutated versions of FLAG-tagged msl-1 were tested for their ability to dimerize with Myc-tagged wild-type msl-1.

(Hallacli et al., 2012)
FBrf0220106-9.coIP.WB
Reference
Hallacli et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
msl-1 

Msl1

bait

FLAG tag

msl-1 

Msl1

prey

HA tag

msl-1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
msl-1 
coiled coil
mutation disrupting interaction
aa 114-145

V114D, M121D, M142D, V145D quadruple mutation

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of SL2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Hallacli et al., 2012)

<symbol> msl-1-XP <ID_as_reported> Msl1 <role> bait <qual/note> FLAG tag

(Hallacli et al., 2012)

<symbol> msl-1-XP <ID_as_reported> Msl1 <role> prey <qual/note> HA tag

(Hallacli et al., 2012)

Dimerization of msl-1 is not affected by mutations that disrupt the binding of msl-1 to msl-2, msl-3 or mof.

(Hallacli et al., 2012)

Mutated versions of FLAG-tagged msl-1 were tested for their ability to dimerize with Myc-tagged wild-type msl-1.

(Hallacli et al., 2012)
External Crossreferences and Linkouts ( 0 )
References (2)