Source was adult female ovary of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

jar coimmunoprecipitates Cam in ovary, but not in testis. This interaction is probably Calcium-dependent, as the interaction is inhibited in the presence of EGTA.

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Two-hybrid system: yeast GAL4-BD/GAL4-AD

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey chemically synthesized.

Cam binds to jar peptides more strongly in the presence of Calcium.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey chemically synthesized.

Both the N-lobe and C-lobe of Cam bind to the jar \'Insert 2\' or \'IQ\' motif peptides.

Interaction in vitro; Cam produced as a recombinant fusion protein in bacterial system; jar peptide chemically synthesized.

Mass spectrometry was used to examine dynamic changes in Cam upon interaction with the binding motifs of jar.