An S120 extract, in which plasma membrane and internal membrane compartments were removed by centrifugation, was subjected to Optiprep density gradient fractionation.

Source was larvae of transgenic fly line; proteins produced from endogenous gene or from tagged transgenic construct.

The association of dally with Rfabg is mediated by both its GPI lipid anchor and glycosylation sites.

Source was larvae of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

The interaction of dally with Rfabg is detectable but reduced when dally lacks a GPI lipid anchor.