Interaction in vitro; protein produced as a recombinant fusion proteins in bacterial system.

Dimerization of the swa alpha-helical coiled-coil domain is stimulated by the addition of ctp.

Interaction in vitro; protein produced as a recombinant fusion proteins in bacterial system.

Dimerization of the swa alpha-helical coiled-coil domain is stimulated by the addition of ctp.

Purified swa protein was cross-linked and the presence of higher order complexes monitored by SDS-PAGE.

Interaction in vitro; protein produced as a recombinant fusion proteins in bacterial system.

swa protein forms dimers, especially at higher protein concentrations, as determined by gel filtration.

Interaction in vitro; protein produced as a recombinant fusion proteins in bacterial system.

swa protein forms dimers with Kd = 4uM, consistent with weak dimeric coiled-coil.

Interaction in vitro; protein produced as a recombinant fusion protein in bacterial system.

Mutation of residues in the swa coiled-coil region can facilitate or prevent swa homodimerization.

Circular dichroism analysis suggests that monomeric swa is disordered, while dimeric swa has a spectrum characteristic of supercoiling.

Interaction in vitro; protein produced as a recombinant fusion protein in bacterial system.

Mutation of residues in the swa coiled-coil region can facilitate or prevent swa homodimerization.