FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
ERR, sima
FlyBase ID
FBig0000097386
Interaction Network
Interactions Browser links
ERR network
sima network
Reported Interactions
FBrf0220675-1.PD.A
Reference
Li et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ERR 

ERR

bait

GST tag

ERR 
ERR 
sima 

dHIFα

prey

[35]S label

sima 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ERR 
ligand binding domain
sufficient binding region
aa 193-496

L193-R496

ERR 
AF2 motif
mutation decreasing interaction
aa 479-491

deletion of AF2 motif

sima 
LXXLL motif
mutation decreasing interaction
aa 1292-1293

L1292A, L1293A double mutant

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Li et al., 2013)
FBrf0220675-2.Y2H
Reference
Li et al., 2013
Description
physical association
Assay
Collection
Source/Stage
organism / late third instar larval stageprepupal stage P3prepupal stage P2prepupal stage P1
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ERR 

ERR

bait

fused to GAL4 DNA-binding domain

ERR 
sima 

sima

prey

fused to GAL4 activation domain

sima 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ERR 
ligand binding domain
sufficient binding region
aa 193-496

L193-R496

sima 
C-terminal region
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Li et al., 2013)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from larval-prepupal cDNA expression library).

(Li et al., 2013)

7 of 20 ERR-interacting clones represented C-terminal fragments of sima (dHIFa).

(Li et al., 2013)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)