FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Orco, Or22a
FlyBase ID
FBig0000097400
Interaction Network
Interactions Browser links
Orco network
Or22a network
Reported Interactions
FBrf0184196-4.BRET
Reference
Neuhaus et al., 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Orco 

DOR83b

bait

Luciferase tag

Or22a 

DOR22a

prey

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was live HEK293 cells; bait produced from transfected construct; prey produced from transfected construct.

(Neuhaus et al., 2005)
FBrf0192657-5.Y2H
Reference
Benton et al., 2006
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Orco 

OR83b

bait

fused to GAL4 DNA-binding domain

Orco 
Or22a 

OR22a

prey

fused to GAL4 activation domain

Or22a 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Orco 
intercellular loop 3
sufficient binding region
aa 412-459
Or22a 
intercellular loop 3
sufficient binding region
aa 305-359
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Benton et al., 2006)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Benton et al., 2006)
FBrf0220621-3.FRET
Reference
German et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Orco 

Orco

bait

CFP tag at intracellular loop 2

Or22a 

Or22a

prey

YFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was baculovirus-infected Sf9 cells; bait produced from transfected construct; prey produced from transfected construct.

(German et al., 2013)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)