Interaction in vitro; bait produced as a recombinant fusion protein in vertebrate cell line; prey produced from transfected construct.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

Sdc ectodomain binds S2 cells expressing Lar, but not to untransfected S2 cells.

Purified Sdc fusion protein was tested for its ability to bind live S2 cells expressing Lar protein.

Interaction in vitro; bait produced as a recombinant fusion protein in vertebrate cell line; prey produced as a recombinant fusion protein in vertebrate cell line.

Lar binds to dlp (Kd = 7.8nM) more strongly than to Sdc (Kd = 12.8nM).

Removal of Sdc glycosylation by mutation or enzymatic treatment disrupts the interaction.