Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Both PHD domains of Pcl are required for interaction with esc.

Two-hybrid system: yeast LexA-BD/B42-AD

Source was embryonic nuclear extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

The 600 kDa PRC2 complex (E(z), esc, Su(z)12, Caf1) is also present in larger 1 MDa complexes that additionally contain Rpd3 and Pcl. The larger 1 MDa PRC2 complex is only observed in 0-16 hr embryos, not in 18-24 hr embryos.

This interaction of Pcl with E(z) is detected only in 0-16 hr embryos, but not in 18-24 hr embryos.

Immunoprecipitation of Pcl from size fractionated extracts containing complexes of about 1 MDa in size.

Source was embryonic nuclear extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

This interaction of E(z) with Pcl is detected only in 0-16 hr embryos, but not in 18-24 hr embryos.

Source was cell extract of S2R+ cell line; proteins produced from transfected construct or endogenous gene.

HGScore = 29.81195