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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000097437
Interaction Network
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0105275-1.Y2H
Reference
Description
physical association
Assay
Collection
Cell line used
Participants
Corresponds to
Reported as
Role
Note
numb 

Numb

bait

fused to LexA DNA-binding domain

numb 
pon 

pon

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
numb 
PTB domain
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

Two-hybrid system: yeast LexA-BD/GAL4-AD

FBrf0105275-2.Y2H
Reference
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
numb 

Numb

bait

fused to LexA DNA-binding domain

numb 
numb 
pon 

PON

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
numb 
PTB domain
sufficient binding region
numb 
PTB domain
mutation disrupting interaction
aa 195

F195V

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Two-hybrid system: yeast LexA-BD/GAL4-AD

FBrf0105275-3.Y2H
Reference
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pon 

PON

bait

fused to LexA DNA-binding domain

pon 
numb 

Numb

prey

fused to GAL4 activation domain

numb 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
numb 
PTB domain
sufficient binding region
pon 
N-terminal region
sufficient binding region
aa 1-222
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Two-hybrid system: yeast LexA-BD/GAL4-AD

FBrf0105275-4.coIP.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
numb 

Numb

bait

[35]S label

pon 

PON

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced and labeled by in vitro translation; prey produced and labeled by in vitro translation.

FBrf0105275-5.coIP.WB
Reference
Description
physical association
Collection
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pon 

PON

bait

Myc tag

numb 

Numb

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

FBrf0187299-3.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
numb 

Numb

bait

MYC tag

pon 

PON

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

A mutant form of numb, numb S52F, bound similar amounts of pon as compared to wild type numb

FBrf0213702-3.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
numb 

numb

bait

Myc tag

pon 

pon

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of HEK293T cell line; bait produced from transfected construct; prey produced from transfected construct.

FBrf0238194-1.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
numb 

Nb

bait

HA tag

numb 
pon 

Pon

prey

FLAG tag

pon 
pon 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
numb 
PTB domain
sufficient binding region
aa 65-203

coordinates relative to numb-PA

pon 
N-terminal domain
sufficient binding region
aa 1-228

coordinates relative to pon-PA

pon 
N-terminal domain
necessary binding region
aa 117-228

coordinates relative to pon-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of HEK293T cell line; bait produced from transfected construct; prey produced from transfected construct.

FBrf0238194-2.PD.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
numb 

Nb

bait

GST tag

numb 
pon 

Pon

prey

FLAG tag

pon 
pon 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
numb 
PTB domain
sufficient binding region
aa 65-203

coordinates relative to numb-PA

pon 
N-terminal domain
sufficient binding region
aa 1-228

coordinates relative to pon-PA

pon 
N-terminal domain
necessary binding region
aa 117-228

coordinates relative to pon-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transfected HEK293T cell extract.

FBrf0238194-3.PD.MW
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pon 

Pon

bait

GST tag

pon 
pon 
pon 
pon 
pon 
pon 
pon 
pon 
pon 
pon 
pon 
pon 
numb 

Nb

prey

His tag

numb 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
numb 
PTB domain
sufficient binding region
aa 65-203

coordinates relative to numb-PA

pon 
A1B3 domain
sufficient binding region
aa 1-228

coordinates relative to pon-PA

pon 
B1B3 domain
sufficient binding region
aa 110-200

coordinates relative to pon-PA

pon 
A1B2 domain
sufficient binding region
aa 1-162

coordinates relative to pon-PA

pon 
A2B3 domain
sufficient binding region
aa 130-200

coordinates relative to pon-PA

pon 
A1B1 domain
sufficient binding region
aa 41-130

coordinates relative to pon-PA

pon 
B1A2 domain
sufficient binding region
aa 110-148

coordinates relative to pon-PA

pon 
A2B2 domain
sufficient binding region
aa 130-162

coordinates relative to pon-PA

pon 
A2 domain residue 139
mutation disrupting interaction
aa 139

N139A, coordinates relative to pon-PA, effect of mutation in the context of pon A2B2 domain binding to numb PTB domain.

pon 
B2 domain residue 154
mutation disrupting interaction
aa 154

Y154E, coordinates relative to pon-PA, effect of mutation in the context of pon A2B2 domain binding to numb PTB domain.

pon 
A3B3 domain
sufficient binding region
aa 157-200

coordinates relative to pon-PA

pon 
A3 domain residue 169
mutation disrupting interaction
aa 169

N169A, coordinates relative to pon-PA, effect of mutation in the context of pon A3B3 domain binding to numb PTB domain.

pon 
B3 domain residue 186
mutation disrupting interaction
aa 186

F186E, coordinates relative to pon-PA, effect of mutation in the context of pon A3B3 domain binding to numb PTB domain.

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

pon as bait brings down more numb than the reciprocal experiment.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

FBrf0238194-4.PD.MW
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
numb 

Nb

bait

GST tag

numb 
pon 

Pon

prey

His tag

pon 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
numb 
PTB domain
sufficient binding region
aa 65-203

coordinates relative to numb-PA

pon 
A1B3 domain
sufficient binding region
aa 1-228

coordinates relative to pon-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

numb as bait brings down less pon than the reciprocal experiment.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

FBrf0238194-5.ITC
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
numb 

Nb

bait
numb 
numb 
numb 
numb 
pon 

Pon

prey
pon 
pon 
pon 
pon 
pon 
pon 
pon 
pon 
pon 
pon 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
numb 
PTB domain
sufficient binding region
aa 65-203

coordinates relative to numb-PA

numb 
PTB domain residue 90
mutation disrupting interaction
aa 90

C90W, coordinates relative to numb-PA, effect of mutation in the context of numb PTB domain binding to pon AB domains

numb 
PTB domain residue 171
mutation disrupting interaction
aa 171

R171A, coordinates relative to numb-PA, effect of mutation in the context of numb PTB domain binding to pon AB domains

numb 
PTB domain residue 192
mutation disrupting interaction
aa 192

G192L or G192D, coordinates relative to numb-PA, effect of mutation in the context of numb PTB domain binding to pon AB domains

pon 
A1B1 domain
sufficient binding region
aa 41-130

coordinates relative to pon-PA, Kd=18.1E-6M

pon 
B1A2 domain
sufficient binding region
aa 110-148

coordinates relative to pon-PA, Kd=8.77E-6M

pon 
B1 domain residue 121
mutation disrupting interaction
aa 121

E121A, coordinates relative to pon-PA, effect of mutation in the context of pon B1A2 domain binding to numb PTB domain.

pon 
A2B2 domain
sufficient binding region
aa 130-162

coordinates relative to pon-PA, Kd=1.8E-6M

pon 
A2 domain residue 139
mutation disrupting interaction
aa 139

N139A, coordinates relative to pon-PA, effect of mutation in the context of pon A2B2 domain binding to numb PTB domain.

pon 
B2 domain residue 154
mutation disrupting interaction
aa 154

Y154E, coordinates relative to pon-PA, effect of mutation in the context of pon A2B2 domain binding to numb PTB domain.

pon 
B2 domain residue 155
mutation disrupting interaction
aa 155

E155A, coordinates relative to pon-PA, effect of mutation in the context of pon A2B2 domain binding to numb PTB domain.

pon 
B2 domain residue 158
mutation disrupting interaction
aa 158

R158A, coordinates relative to pon-PA, effect of mutation in the context of pon A2B2 domain binding to numb PTB domain.

pon 
A3B3 domain
sufficient binding region
aa 157-200

coordinates relative to pon-PA, Kd=12.2E-6M

pon 
B3 domain residue 187
mutation disrupting interaction
aa 187

E187A, coordinates relative to pon-PA, effect of mutation in the context of pon A3B3 domain binding to numb PTB domain.

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

FBrf0238194-6.XC
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
numb 

Nb

neutral component
numb 

Nb

neutral component
numb 
numb 

Nb

neutral component
numb 
pon 

Pon

neutral component
pon 
pon 
pon 

Pon

neutral component
pon 

Pon

neutral component
pon 
pon 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
numb 
PTB domain
sufficient binding region
aa 65-203

coordinates relative to numb-PA

pon 
A2B2 domain
sufficient binding region
aa 130-162

coordinates relative to pon-PA

pon 
B1A2 domain
sufficient binding region
aa 110-148

coordinates relative to pon-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The numb PTB domain and pon AB domain form a dimer of dimers.

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

FBrf0238194-7.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pon 

Pon

bait

FLAG tag

pon 
pon 
pon 
pon 
numb 

Nb

prey

HA tag

numb 
numb 
numb 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
numb 
PTB domain residue 90
mutation decreasing interaction
aa 90

C90W, coordinates relative to numb-PA

numb 
PTB domain residue 192
mutation decreasing interaction
aa 192

G192D, coordinates relative to numb-PA

numb 
PTB domain residues 90,192
mutation disrupting interaction
aa 90,192

C90W,G192D, coordinates relative to numb-PA

pon 
AB domain residues 139,154
mutation decreasing interaction
aa 139,154

N139A,Y154E, coordinates relative to pon-PA

pon 
AB domain residues 169,186
mutation decreasing interaction
aa 169,186

N169A,F186E, coordinates relative to pon-PA

pon 
AB domain residues 139,154,169,186
mutation decreasing interaction
aa 139,154,169,186

N139A,Y154E,N169A,F186E, coordinates relative to pon-PA

pon 
AB domain residues 57,120,139,154,169,186
mutation disrupting interaction
aa 57,120,139,154,169,186

N57A,F120E,N139A,Y154E,N169A,F186E, coordinates relative to pon-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of HEK293T cell line; bait produced from transfected construct; prey produced from transfected construct.

FBrf0238194-9.CS.MW
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
numb 

Nb

neutral component
numb 

Nb

neutral component
numb 
numb 

Nb

neutral component
numb 
pon 

Pon

neutral component
pon 
pon 

Pon

neutral component
pon 

Pon

neutral component
pon 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
numb 
PTB domain
sufficient binding region
aa 65-203

coordinates relative to numb-PA

pon 
A2B2 domain
sufficient binding region
aa 130-162

coordinates relative to pon-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The estimated molecular weight of the numb PTB domain bound to pon A2B2 domain is consistent with a dimer of dimers.

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (4)