FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
cnn, γTub23C
FlyBase ID
FBig0000097470
Interaction Network
Interactions Browser links
cnn network
γTub23C network
Reported Interactions
FBrf0162192-4.PD.WB
Reference
Terada et al., 2003
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
cnn 

CNN

bait

His tag

cnn 
γTub23C 

γ-tubulin

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
cnn 
N-terminal region
sufficient binding region
aa 1-705
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Cell extracts were prepared from mitotic S2 cells.

(Terada et al., 2003)

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from S2 cell extract.

(Terada et al., 2003)

cnn may interact with γTub indirectly, as this interaction could not be verified by yeast two hybrid or other methods.

(Terada et al., 2003)
FBrf0219185-28.coIP.WB
Reference
Habermann et al., 2012
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 1embryonic stage 6blastoderm stageembryonic stage 5embryonic stage 3embryonic cycle 6embryonic cycle 8embryonic cycle 5embryonic cycle 7embryonic cycle 4embryonic cycle 3embryonic stage 4embryonic stage 2embryonic cycle 9
Cell line used
Participants
Corresponds to
Reported as
Role
Note
cnn 

cnn

bait
γTub23C 

γ-Tub

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was purified embryonic centrosomes of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Habermann et al., 2012)

Centrosomes were isolated from highly mitotic syncytial embryos and subjected to cnn protein affinity purification.

(Habermann et al., 2012)
FBrf0239562-9.PD.WB
Reference
Tovey et al., 2018
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
cnn 

Cnn

bait

MBP tag

cnn 
cnn 
γTub23C 

γ-tubulin

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
cnn 
interaction domain
sufficient binding region
aa 1-197

coordinates relative to cnn-PG

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type embryonic extract.

(Tovey et al., 2018)
FBrf0249106-9.PD.MS
Reference
Tovey et al., 2021
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
cnn 

Cnn

bait

MBP tag

cnn 
γTub23C 

γTub23C

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
cnn 
cnn-T-N-terminal domain residues 1-197
sufficient binding region
aa 1-197

coordinates relative to cnn-PD

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type embryonic extract.

(Tovey et al., 2021)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (4)