FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
polo, mtrm
FlyBase ID
FBig0000097489
Interaction Network
Interactions Browser links
polo network
mtrm network
Reported Interactions
FBrf0202869-1.coIP.WB
Reference
Xiang et al., 2007
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mtrm 

Mtrm

bait
mtrm 
polo 

Polo

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
mtrm 
STP domain residue 40
mutation disrupting interaction
aa 40

T40A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult ovaries of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Xiang et al., 2007)
FBrf0202869-2.CH.MP
Reference
Xiang et al., 2007
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mtrm 

Mtrm

bait

FLAG tag

polo 

Polo

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult ovaries of transgenic fly line; bait produced tagged transgenic construct; prey produced from endogenous gene.

(Xiang et al., 2007)
FBrf0202869-3.coIP.WB
Reference
Xiang et al., 2007
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
polo 

Polo

bait

GFP tag

mtrm 

Mtrm

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult ovaries of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Xiang et al., 2007)
FBrf0202869-4.coIP.WB
Reference
Xiang et al., 2007
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
polo 

Polo

bait
mtrm 

Mtrm

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult ovaries of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Xiang et al., 2007)
FBrf0221228-1.coIP.WB
Reference
Bonner et al., 2013
Description
physical association
Assay
Collection
Source/Stage
ovary / adult stagefemale
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mtrm 

Mtrm

bait

FLAG tag

mtrm 
mtrm 
mtrm 
polo 

Polo

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
mtrm 
N-terminal region
necessary binding region
aa 2-54
mtrm 
SAM domain
necessary binding region
aa 154-217
mtrm 
phosphorylation site
mutation disrupting interaction
aa 40aa 48aa 52

T40A

S48A

S52A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult female ovary of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Bonner et al., 2013)

mtrm binds polo via a non-canonical site.

(Bonner et al., 2013)
FBrf0221228-2.Y2H
Reference
Bonner et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
polo 

Polo

bait

fused to GAL4 DNA-binding domain

mtrm 

Mtrm

prey

fused to GAL4 activation domain

mtrm 
mtrm 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
mtrm 
phosphorylation site
mutation disrupting interaction
aa 48aa 52

S48A

S52A

mtrm 
SAM domain
necessary binding region
aa 154-217
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Bonner et al., 2013)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Bonner et al., 2013)

Mutations in the polo-box domain (H518, K520M) do not disrupt binding to mtrm.

(Bonner et al., 2013)
FBrf0221228-3.FCS
Reference
Bonner et al., 2013
Description
physical association
Assay
Collection
Source/Stage
ovary / adult stagefemale
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mtrm 

Mtrm

bait

mCherry tag

mtrm 
mtrm 
polo 

Polo

prey

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
mtrm 
N-terminal region
necessary binding region
aa 2-54
mtrm 
SAM domain
necessary binding region
aa 154-217
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult female ovary of transgenic fly line; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.

(Bonner et al., 2013)

Mutations in the polo-box domain (H518, K520M) do not disrupt binding to mtrm.

(Bonner et al., 2013)
FBrf0221228-4a.coIP.WB
Reference
Bonner et al., 2013
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
polo 

Polo

bait

GFP tag

mtrm 

Mtrm

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult female ovary of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Bonner et al., 2013)

Mutations in the polo-box domain (H518, K520M) do not disrupt binding to mtrm.

(Bonner et al., 2013)
FBrf0241273-1.coIP.WB
Reference
Bonner and Hawley, 2019
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mtrm 

Mtrm

bait

FLAG tag

polo 

Polo

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was ovaries of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Bonner and Hawley, 2019)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)