High speed supernatant of S3 cell lysates was fractionated over a DEAE Sephacel column, concentrated by ammonium sulfate precipitation, and resolubilized for gel filtration. This yielded a purified fraction containing both the 234 kDa and 226 kDa spectrin subunits.
Source was S3 cell line; bait produced from endogenous gene; prey produced from endogenous gene.
Interaction in vitro; bait produced by in vitro translation; prey produced and labeled by in vitro translation.
Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
"α 0-1" construct, coordinates relative to α-Spec-PA
"β 16-17" construct, coordinates relative to β-Spec-PA
Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.
"α 0-1" construct, coordinates relative to α-Spec-PA
"β 16-17" construct, coordinates relative to β-Spec-PA
R22S, coordinates relative to α-Spec-PA
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.
Kd = 14 nM (at 23oC).
Source was adult heads of transgenic fly line; bait produced from endogenous gene; prey produced from endogenous gene.
Positive control; transgenic flies contain only GAL4 without a UAS construct.
aa 1-44 not necessary for interaction. Isoform not specified.
Source was adult heads of transgenic fly line; bait produced from endogenous gene; prey produced from tagged transgenic construct.
