Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from ovary cDNA expression library).

The five strongest pll-interacting hits in this screen represented Pli cDNAs.

Two-hybrid system: yeast LexA-BD/B42-AD

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

A kinase-dead version of pll does not interact with Pli. However, pll does not phosphorylate Pli in vitro.

Two-hybrid system: yeast LexA-BD/B42-AD

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

Pli binds to pll but not tub in this assay.