FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Pak3
FlyBase ID
FBig0000097564
Interaction Network
Interactions Browser links
Pak3 network
Reported Interactions
FBrf0187582-4.ENZ.A
Reference
Mentzel and Raabe, 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Pak3 

DmPAK3

self

HA tag, [32]P label

Pak3 

DmPAK3

self

HA tag, [32]P label

Pak3 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Pak3 
kinase domain
mutation disrupting interaction
aa 322

K322A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; enzyme derived from transfected HEK293 cell line extract.

(Mentzel and Raabe, 2005)

Pak3 is autophosphorylated.

(Mentzel and Raabe, 2005)

Pak3 kinase activity is stimulated by activated Rac1.

(Mentzel and Raabe, 2005)
FBrf0187582-5.coIP.WB
Reference
Mentzel and Raabe, 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Pak3 

DmPAK3

bait

HA tag

Pak3 

DmPAK3

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Pak3 self associates.

(Mentzel and Raabe, 2005)

Source was cell extract of HEK293 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Mentzel and Raabe, 2005)
FBrf0207349-10.ENZ.A
Reference
Mentzel et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Pak3 

DmPAK3

self

Myc tag, P[32] label

Pak3 

DmPAK3

self

Myc tag, P[32] label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; protein immunopurified from transfected HEK293 cell.

(Mentzel et al., 2009)

In vitro kinase assay. Coimmunopurification of CkIIβ, CkIIβ2, or Ssl with Pak3 inhibits Pak3 autophosphorylation.

(Mentzel et al., 2009)
External Crossreferences and Linkouts ( 0 )
References (2)