FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
His3, Su(z)12
FlyBase ID
FBig0000097587
Interaction Network
Interactions Browser links
His3 network
Su(z)12 network
Reported Interactions
FBrf0219425-7.XL.MW
Reference
Yuan et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
His3 

H3

bait

DOPA and biotin conjugated

His3 
His3 
Su(z)12 

Su(z)12

prey
Su(z)12 
Su(z)12 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Su(z)12 
VEFS-BOX
sufficient binding region
aa 507-630
Su(z)12 
conserved acidic residues
mutation disrupting interaction
aa 548-552

E548A, E550A ,D552A

His3 
region
sufficient binding region
aa 35-42
His3 
region
mutation disrupting interaction
aa 40

R40A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

DOPA-conjugated His3 peptide was mixed with purified PRC2 complex and cross-linked by periodate treatment. Labeled PRC2 components were assessed by SDS-PAGE and visualization of the His3 peptide\'s biotin tag .

(Yuan et al., 2012)

Interaction in vitro; bait synthesized in vitro; prey produced as a recombinant fusion protein in baculovirus system.

(Yuan et al., 2012)
External Crossreferences and Linkouts ( 0 )
References (1)