Interaction in vitro; bait produced as a recombinant fusion protein in COS cell system; prey produced as a recombinant fusion protein in COS cell system.

Binding requires divalent cations (Ca[++] or Mg[++]).

Transmembrane bait protein was obtained from crude membrane preparations of transfected cells. Prey protein was obtained from cell culture supernatants of transfected cells. Proteins were mixed in vitro.

Source was live S2 cells; proteins produced from transfected constructs.

Physical interaction of proteins expressed on the surface of cells is inferred from the aggregation of live cells.

Assay: cell aggregation

Interaction in vitro; bait derived from conditioned medium of transfected S2 cell line; prey derived from conditioned medium of transfected S2 cell line.

Positive control.

Interaction in vitro; bait derived from conditioned medium of transfected S2 cell line; prey derived from conditioned medium of transfected S2 cell line.

Positive control.

Interaction in vitro; bait produced as a recombinant fusion protein in HEK293 cells; prey produced as a recombinant fusion protein in HEK293 cells.

Kd = 141nM.

Positive control. Kd = 7.4uM

Interaction in vitro; bait produced as a recombinant fusion protein in HEK293T cell system; prey source unspecified.

Interaction in COS-7 cell system; ligand purified as recombinant fusion protein from conditioned medium of transfected HEK 293T cells; receptor produced from transfected construct.

Interaction inferred from the ability of ligand to induce a morphological collapse in a heterologous system expressing the receptor. Coexpression of Sema1b with PlexA prevents Sema1a induced collapse.