FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
trbl, slbo
FlyBase ID
FBig0000097595
Interaction Network
Interactions Browser links
trbl network
slbo network
Reported Interactions
FBrf0130050-1.coIP.WB
Reference
Rorth et al., 2000
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
trbl 

trbl

bait

T7 tag

slbo 

slbo

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Rorth et al., 2000)

trbl stimulates slbo ubiquitination and proteolysis.

(Rorth et al., 2000)
FBrf0220729-1.Y2H
Reference
Masoner et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
trbl 

Trbl

bait

fused to GAL4 DNA-binding domain

trbl 
slbo 

Slbo

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
trbl 
kinase domain
mutation disrupting interaction
aa 264

D264N, \'D/NLK\' mutant

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Masoner et al., 2013)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Masoner et al., 2013)
FBrf0220729-2.Y2H
Reference
Masoner et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
slbo 

Slbo

bait

fused to GAL4 DNA-binding domain

trbl 

Trbl

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Masoner et al., 2013)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Masoner et al., 2013)
FBrf0237536-4.Y2H
Reference
Fischer et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
trbl 

Trbl

bait

fused to GAL4 DNA-binding domain

trbl 
slbo 

Slbo

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
trbl 
interaction domain residue 141
mutation decreasing interaction
aa 141

R141Q

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Fischer et al., 2017)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Fischer et al., 2017)
FBrf0262229-3.Y2H
Reference
Fischer et al., 2025
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
slbo 

Slbo

bait

fused to GAL4 DNA-binding domain

trbl 

trbl

prey

fused to GAL4 activation domain

trbl 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
trbl 
DLK motif residue 264
mutation decreasing interaction
aa 284

D264N ; coordinates relative to trbl-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Fischer et al., 2025)

Two-hybrid system: Yeast GAL4-BD/GAL4-AD. trblSLE/G-trbl bait-prey co-transformants grew on higher concentrations of the 3-AT His3 inhibitor than the trbl-trbl bait-prey co-transformants.

(Fischer et al., 2025)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (4)