FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
E(spl)m3-HLH, sc
FlyBase ID
FBig0000097619
Interaction Network
Interactions Browser links
E(spl)m3-HLH network
sc network
Reported Interactions
FBrf0099085-8.Y2H
Reference
Alifragis et al., 1997
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
E(spl)m3-HLH 

m3

bait

fused to LexA DNA-binding domain

E(spl)m3-HLH 
sc 

Sc

prey

fused to VP16 activation domain

sc 
sc 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
E(spl)m3-HLH 
bHLH domain
sufficient binding region
aa 1-77
sc 
bHLH domain
sufficient binding region
aa 1-178
sc 
bHLH domain
mutation disrupting interaction
aa 340

S340D

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Alifragis et al., 1997)

E(spl)m3-HLH interacts with sc, ac, ase and gro, but not with da or run, in this assay.

(Alifragis et al., 1997)

Two-hybrid system: yeast GAL4-BD/VP16-AD

(Alifragis et al., 1997)
FBrf0102176-5.Y2H
Reference
Poortinga et al., 1998
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
E(spl)m3-HLH 

m3

bait

fused to LexA DNA-binding domain

sc 

Scute

prey

fused to VP16 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Poortinga et al., 1998)

sc interacts with E(spl)m3-HLH, E(spl)m4-BFM, E(spl)mβ-HLH and E(spl)mγ-HLH, but not h, dpn, E(spl)m5-HLH, E(spl)m8-HLH or E(spl)mδ-HLH.

(Poortinga et al., 1998)

Two-hybrid system: yeast LexA-BD/VP16-AD

(Poortinga et al., 1998)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)