FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Apc2
FlyBase ID
FBig0000097698
Interaction Network
Interactions Browser links
Apc2 network
Reported Interactions
FBrf0213457-1.coIP.WB
Reference
Zhou et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Apc2 

APC2

bait

mCherry tag

Apc2 

APC2

prey
Apc2 
Apc2 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Apc2 
Arm-domain
sufficient binding region
aa 51-424

coordinates relative to Apc2-PA

Apc2 
N terminal domain
sufficient binding region
aa 1-17

coordinates relative to Apc2-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Zhou et al., 2011)
FBrf0218500-1.coIP.WB
Reference
Roberts et al., 2012
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Apc2 

APC2

bait

GFP tag

Apc2 

APC2

prey
Apc2 
Apc2 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Apc2 
Arm repeats
sufficient binding region
Apc2 
Arm repeats
necessary binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Roberts et al., 2012)

The Arm repeats are necessary and sufficient for Apc2 self-association.

(Roberts et al., 2012)
FBrf0226638-1.coIP.WB
Reference
Kunttas-Tatli et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Apc2 

Apc2

bait

mCherry tag

Apc2 

Apc2

prey
Apc2 
Apc2 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Apc2 
self-association domain
necessary binding region
aa 70-100

coordinates relative to Apc2-PA

Apc2 
self-association domain
mutation disrupting interaction
aa 81,84,94,98

coordinates relative to Apc2-PA, L81P,L84P,L94P,L98P

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Kunttas-Tatli et al., 2014)
FBrf0226638-3.Y2H
Reference
Kunttas-Tatli et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Apc2 

Apc2

bait

fused to GAL4 DNA-binding domain

Apc2 

Apc2

prey

fused to GAL4 activation domain

Apc2 
Apc2 
Apc2 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Apc2 
N-terminal region
sufficient binding region
aa 1-490

coordinates relative to Apc2-PA

Apc2 
self-association domain
necessary binding region
aa 70-100

coordinates relative to Apc2-PA

Apc2 
self-association domain
mutation disrupting interaction
aa 81,84,94,98

coordinates relative to Apc2-PA, L81P,L84P,L94P,L98P

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Kunttas-Tatli et al., 2014)
External Crossreferences and Linkouts ( 0 )
References (3)