FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
apolpp, Nrx-1
FlyBase ID
FBig0000097721
Interaction Network
Interactions Browser links
apolpp network
Nrx-1 network
Reported Interactions
FBrf0220614-1.Y2H
Reference
Tian et al., 2013
Description
physical association
Assay
Collection
Source/Stage
testis / adult stagemale
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Nrx-1 

Nrx

bait

fused to GAL4 DNA-binding domain

Nrx-1 
apolpp 

RFABG

prey

fused to GAL4 activation domain

apolpp 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Nrx-1 
intracellular domain
sufficient binding region
aa 1716–1838
apolpp 
ApoL I region
sufficient binding region
aa 1090-1480aa 1390-1871

\'G5\'

\'K1\'

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Tian et al., 2013)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from testis cDNA expression library).

(Tian et al., 2013)

Rfabg is a precursor protein that is cleaved to generate ApoLI and ApoLII. Nrx-1-interacting clones of Rfabg recovered in the two-hybrid screen correspond to the \'ApoLI\' region.

(Tian et al., 2013)
FBrf0220614-2.PD.WB
Reference
Tian et al., 2013
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
apolpp 

RFABG

bait

MBP tag

apolpp 
Nrx-1 

Nrx

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
apolpp 
ApoL I region
sufficient binding region
aa 1390-1480
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type adult head extract.

(Tian et al., 2013)
FBrf0220614-3.coIP.WB
Reference
Tian et al., 2013
Description
physical association
Assay
Collection
Source/Stage
retina / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Nrx-1 

nrx

bait
apolpp 

ApoL I

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult retina of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Tian et al., 2013)
FBrf0220614-4.coIP.WB
Reference
Tian et al., 2013
Description
physical association
Assay
Collection
Source/Stage
retina / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
apolpp 

ApoL I

bait
Nrx-1 

Nrx

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult retina of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Tian et al., 2013)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)