FB2025_01 , released February 20, 2025
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Nek2, dsh
FlyBase ID
FBig0000097742
Interaction Network
Interactions Browser links
Nek2 network
dsh network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0221461-1.coIP.MS
Reference
Schertel et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Nek2 

Nek2

bait

HA tag

dsh 

Dsh

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of Kc cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Schertel et al., 2013)
FBrf0221461-2.coIP.WB
Reference
Schertel et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Nek2 

Nek2

bait

HA tag

dsh 

Dsh

prey
dsh 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
dsh 
central region
sufficient binding region
aa 166-382
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of Kc cell line; bait produced from transfected construct; prey produced from transfected construct.

(Schertel et al., 2013)
FBrf0221461-3.ENZ.A
Reference
Description
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Nek2 

Nek2

enzyme

HA tag

Nek2 

Nek2

enzyme

HA tag

dsh 

Dsh

enzyme target

γ-[32]P label

dsh 

Dsh

enzyme target

γ-[32]P label

dsh 
dsh 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)