Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

Two-hybrid system: yeast GAL4-BD/GAL4-AD

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

Two-hybrid system: yeast GAL4-BD/GAL4-AD

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

Extracts of membrane vesicles were used for immunoprecipitation.

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

Extracts of membrane vesicles were used for immunoprecipitation.

Source was cell extract of SL2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

Drosophila Ote protein is phosphorylated in vitro by human Aurora-A kinase.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

Phosphmimetic mutations of Ote at Ser50 amd Ser54 negatively regulates its interaction with Lam.