Source was adult heads of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.
Two isoforms of Fmr1 were characterized and tested, one with and one without the aa sequence VSA. No difference in homodimerization was detected.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.
Two isoforms of Fmr1 were characterized and tested, one with and one without the aa sequence VSA. No difference in homodimerization was detected.
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.
Positive control.
Source was larval brain of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.
