FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Trl, mod(mdg4)
FlyBase ID
FBig0000097835
Interaction Network
Interactions Browser links
Trl network
mod(mdg4) network
Reported Interactions
FBrf0180528-8.Y2H
Reference
Pai et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mod(mdg4) 

Mod(mdg4)2.2

bait

fused to GAL4 DNA-binding domain

Trl 

GAGA

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Pai et al., 2004)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Pai et al., 2004)
FBrf0180644-1.coIP.WB
Reference
Melnikova et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Schneider's-line-2
Participants
Corresponds to
Reported as
Role
Note
mod(mdg4) 

Mod(mdg4)

bait
Trl 

GAF

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extracts of SL2 cells; bait produced from endogenous gene; prey produced from endogenous gene.

(Melnikova et al., 2004)
FBrf0180644-2.coIP.WB
Reference
Melnikova et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Schneider's-line-2
Participants
Corresponds to
Reported as
Role
Note
Trl 

GAF

bait
mod(mdg4) 

Mod(mdg4)

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extracts of SL2 cells; bait produced from endogenous gene; prey produced from endogenous gene.

(Melnikova et al., 2004)
FBrf0180644-4.PD.A
Reference
Melnikova et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Trl 

GAF

bait

His-tag

mod(mdg4) 

mod(mdg4)

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The Mod(mdg4)-PT interacts with the Trl-519 isoform; multiple isoforms of Trl are 519 aa.

(Melnikova et al., 2004)

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Melnikova et al., 2004)
FBrf0180644-5.PD.A
Reference
Melnikova et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mod(mdg4) 

mod(mdg4)

prey

Gst-tag

Trl 
Trl 
Trl 

GAF

prey

[35]S label

Trl 
Trl 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Trl 
interaction domain
sufficient binding region
aa 1-425aa 120-519

Q-rich region removed; interaction slightly reduced relative to full-length

BTB region removed; interaction strongly reduced relative to full-length

Trl 
BTB interaction domain
sufficient binding region
aa 1-150
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

When the Q-rich and BTB domains are both removed the interaction is abolished.

(Melnikova et al., 2004)

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Melnikova et al., 2004)
FBrf0180644-6.Y2H
Reference
Melnikova et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mod(mdg4) 

Mod(mdg4)

bait

mod(mdg4) fused to the DNA binding domain (BD) of Gal4

mod(mdg4) 
Trl 

GAF

prey

Trl fused to the Gal4 activation domain

Trl 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Trl 
interaction domain
sufficient binding region
aa 1-413

mutation decreases interaction.

mod(mdg4) 
BTB domain
necessary binding region
aa 1-106

coordinated relative to mod(mdg4)-PT.

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein.

(Melnikova et al., 2004)

Two-hybrid system: yeast GAL4-BD/GAL4-AD.

(Melnikova et al., 2004)
FBrf0215054-18.Y2H
Reference
Bonchuk et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Trl 

GAF

bait

fused to GAL4 DNA-binding domain

Trl 
Trl 
mod(mdg4) 

Mod(mdg4)

prey

fused to GAL4 activation domain

mod(mdg4) 
mod(mdg4) 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Trl 
BTB domain
sufficient binding region
aa 1-131
mod(mdg4) 
BTB domain
sufficient binding region
aa 1-119
Trl 
N-terminal domain
necessary binding region
aa 2-13
mod(mdg4) 
N-terminal domain
necessary binding region
aa 1-18
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Bonchuk et al., 2011)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Bonchuk et al., 2011)
FBrf0215054-24.Y2H
Reference
Bonchuk et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mod(mdg4) 

Mod(mdg4)

bait

fused to GAL4 DNA-binding domain

mod(mdg4) 
mod(mdg4) 
Trl 

GAF

prey

fused to GAL4 activation domain

Trl 
Trl 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Trl 
BTB domain
sufficient binding region
aa 1-131
mod(mdg4) 
BTB domain
sufficient binding region
aa 1-119
mod(mdg4) 
N-terminal domain
necessary binding region
aa 1-18
Trl 
N-terminal domain
necessary binding region
aa 2-13
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Bonchuk et al., 2011)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Bonchuk et al., 2011)
FBrf0235066-14.coIP.WB
Reference
Lomaev et al., 2017
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Trl 

GAF

bait
mod(mdg4) 

Mod(mdg4)

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Lomaev et al., 2017)
FBrf0235066-27.coIP.MS
Reference
Lomaev et al., 2017
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Trl 

GAF

bait
mod(mdg4) 

Mod(mdg4)

prey

16 peptides identified

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Lomaev et al., 2017)
FBrf0260563-14.Y2H
Reference
Bonchuk et al., 2024
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mod(mdg4) 

mod(mdg4)

bait

fused to GAL4 DNA-binding domain

mod(mdg4) 
Trl 

GAF

prey

fused to GAL4 activation domain

Trl 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Trl 
TTK-type BTB domain
sufficient binding region
aa 1-125
mod(mdg4) 
TTK-type BTB domain
sufficient binding region
aa 1-77
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Bonchuk et al., 2024)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Bonchuk et al., 2024)
FBrf0260563-15.Y2H
Reference
Bonchuk et al., 2024
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Trl 

GAF

bait

fused to GAL4 DNA-binding domain

Trl 
mod(mdg4) 

mod(mdg4)

prey

fused to GAL4 activation domain

mod(mdg4) 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Trl 
TTK-type BTB domain
sufficient binding region
aa 1-125
mod(mdg4) 
TTK-type BTB domain
sufficient binding region
aa 1-77
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Bonchuk et al., 2024)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Bonchuk et al., 2024)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (5)