FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Scm
FlyBase ID
FBig0000097839
Interaction Network
Interactions Browser links
Scm network
Reported Interactions
FBrf0098870-1.Y2H
Reference
Peterson et al., 1997
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Scm 

Scm

bait

fused to LexA DNA-binding domain

Scm 

Scm

prey

fused to GAL4 activation domain

Scm 
Scm 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Scm 
SPM domain
sufficient binding region
aa 767-877
Scm 
SPM domain
necessary binding region
aa 797-877
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast LexA-BD/GAL4-AD

(Peterson et al., 1997)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Peterson et al., 1997)
FBrf0098870-3.PD.A
Reference
Peterson et al., 1997
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Scm 

Scm

bait

GST tag

Scm 

Scm

prey

[35]S label

Scm 
Scm 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Scm 
SPM domain
sufficient binding region
aa 797-877
Scm 
SPM domain
necessary binding region
aa 797-877
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Peterson et al., 1997)
FBrf0179383-1.Y2H
Reference
Peterson et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Scm 

Scm

bait

fused to LexA DNA-binding domain

Scm 

Scm

prey

fused to GAL4 activation domain

Scm 
Scm 
Scm 
Scm 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Scm 
SPM domain
sufficient binding region
aa 767-877
Scm 
SPM domain
necessary binding region
aa 806-877aa 823-877

\'W1stop\'

\'A18fs\'

Scm 
SPM domain
mutation disrupting interaction
aa 809aa 821aa 829aa 834aa 836aa 847aa 851

\'E4K\'

\'S16P\'

\'F24S\', \'F24Y\'

\'I29T\'

\'G31D\' or \'G31S\'

\'M42R\'

\'M46R\'

Scm 
SPM domain
mutation decreasing interaction
aa 835aa 839aa 848aa 853aa 855aa 856aa 860

\'D30G\'

\'L34S\'

\'M43Δ\'

\'L48P\' or \'L48Q\'

\'L50Q\'

\'G51R\'

\'K55E\'

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Peterson et al., 2004)

Two-hybrid system: yeast LexA-BD/GAL4-AD

(Peterson et al., 2004)

Scm was mutagenized in vitro and screened for mutations that disrupt binding to Scm or ph-p.

(Peterson et al., 2004)
FBrf0179383-3.PD.A
Reference
Peterson et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Scm 

SCM

bait

GST tag

Scm 

SCM

prey

[35]S label

Scm 
Scm 
Scm 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Scm 
SPM domain
sufficient binding region
aa 767-877
Scm 
SPM domain
mutation disrupting interaction
aa 834aa 836aa 851aa 860

\'I29T\'

\'G31D\'

\'M46R\'

\'K55E\'

Scm 
SPM domain
mutation decreasing interaction
aa 848

\'M43Δ\'

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Peterson et al., 2004)
External Crossreferences and Linkouts ( 0 )
References (2)