FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
mbt
FlyBase ID
FBig0000097885
Interaction Network
Interactions Browser links
mbt network
Reported Interactions
FBrf0155712-3.ENZ.A
Reference
Schneeberger and Raabe, 2003
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mbt 

Mbt

self

[32]P label

mbt 

Mbt

self

[32]P label

mbt 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
mbt 
kinase domain
mutation disrupting interaction
aa 525

T525A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; enzyme derived from HEK293 cell extract.

(Schneeberger and Raabe, 2003)

mbt autophosphorylates itself.

(Schneeberger and Raabe, 2003)

Activated Cdc42 does not affect mbt\'s kinase activity.

(Schneeberger and Raabe, 2003)

mbt was immunoprecipitated and tested for kinase activity in vitro.

(Schneeberger and Raabe, 2003)
FBrf0195244-1.ENZ.A
Reference
Menzel et al., 2007
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mbt 

Mbt

self

HA tag, P[32] label

mbt 

Mbt

self

HA tag, P[32] label

mbt 
mbt 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
mbt 
kinase defective mbt
mutation disrupting interaction
aa 525

T525A

mbt 
catalytic loop
mutation increasing interaction
aa 492,521

S492N,S521E

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; enzyme produced as a recombinant fusion protein in HEK293 cells; enzyme target produced as a recombinant fusion protein in bacterial system.

(Menzel et al., 2007)
External Crossreferences and Linkouts ( 0 )
References (2)