FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Rga, Rcd-1
FlyBase ID
FBig0000097911
Interaction Network
Interactions Browser links
Rga network
Rcd-1 network
Reported Interactions
FBrf0221179-14.coIP.WB
Reference
Bawankar et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Rga 

NOT2

bait

GFP tag

Rcd-1 

CAF40

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Bawankar et al., 2013)

Rcd-1 (CAF40) preferentially associates with Not1 over other components of the CCR-NOT complex (Rga, Not3, Pop2 and twin).

(Bawankar et al., 2013)
FBrf0257826-12.CH.MW
Reference
Pekovic and Wahle, 2024
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rga 

Not2

neutral component
Rga 

Not2

neutral component
Rga 

Not2

neutral component
Rcd-1 

Caf40

neutral component
Rcd-1 

Caf40

neutral component
Rcd-1 

Caf40

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as a recombinant fusion proteins in baculovirus and insect cell system.

(Pekovic and Wahle, 2024)

Hexameric CCR4-NOT-mini complex captured as 15 pairwise interactions.

(Pekovic and Wahle, 2024)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)