FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Sema1a, RhoGAPp190
FlyBase ID
FBig0000097932
Interaction Network
Interactions Browser links
Sema1a network
RhoGAPp190 network
Reported Interactions
FBrf0219982-3.coIP.WB
Reference
Jeong et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
RhoGAPp190 

p190

bait

HA tag

Sema1a 

Sema-1a

prey

Myc tag

Sema1a 
Sema1a 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Sema1a 
intracellular domain
sufficient binding region
SFPSSCRIQQEPKLLPQVEEVTYAEPVLLPQPPPPNKMHSPKNTLRKPPMHQMHQGPNSE
Sema1a 
intracellular domain
mutation decreasing interaction
CRIQ

mutation to Gly residues weakens interaction

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

(Jeong et al., 2012)

pbl interacts strongly with Sema-1a, but only weakly with plexA or plexB, and not at all with otk.

(Jeong et al., 2012)
FBrf0219982-7.coIP.WB
Reference
Jeong et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
Sema1a 

Sema-1a

bait
RhoGAPp190 

RhoGAPp190

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

(Jeong et al., 2012)

pbl and RhoGAPp190 compete for binding to Sema-1a.

(Jeong et al., 2012)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)