Binding still occurred when the protein lacked the Ring domain: Δneur
Deletion of the Ring domain did not affect binding. anti-Neur antiserum was able to specifically co- precipitate with full-length Ser protein, as well as one consistent with the predicted size for lacking part of the extracellular domain (truncated Ser)
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
Binding still occurred when the protein lacked the Ring domain: ΔRneur
Source was larval disk/CNS complexes of transgenic fly line; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.
Source was larvae of transgenic fly line; bait produced from transgenic construct; prey produced from transgenic construct.
Source was cell extract of Kc cell line; bait produced from transfected construct; prey produced from transfected construct.
