FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Spt5, msl-1
FlyBase ID
FBig0000098014
Interaction Network
Interactions Browser links
Spt5 network
msl-1 network
Reported Interactions
FBrf0220177-1.PD.WB
Reference
Prabhakaran and Kelley, 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Spt5 

SPT5

bait

MBP tag

Spt5 
Spt5 
msl-1 

MSL1

prey

GST label

msl-1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
msl-1 
PEHE domain
sufficient binding region
aa 751-1039
Spt5 
N-terminal region
sufficient binding region
aa 112-393
Spt5 
middle region
sufficient binding region
aa 389-733
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey as a recombinant fusion protein in bacterial system.

(Prabhakaran and Kelley, 2012)

Purified msl-1 PEHE domain interacts with N-terminal or middle fragments of Spt5.

(Prabhakaran and Kelley, 2012)

Attempts to purify intact msl-1-containing MSL complex did not recover Spt5.

(Prabhakaran and Kelley, 2012)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)