Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.
Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.
Bound RNA identified by microarray, defined as having over five-fold enrichment in immunoprecipitates compared to negative controls, with FDR of 5%.
Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
Bound RNA identified by microarray, defined as having over two-fold enrichment in immunoprecipitates compared to negative controls, with FDR of 5%.
