Interaction in vitro; proteins produced by in vitro transcription/translation.

hth and Abd-B, in various combinations, were mixed with [32]P labeled DNA. DNA-protein complexes observed by EMSA, corresponding to Abd-B-hth heterodimers, were used to deduce protein-protein interactions.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

Binding of hth to Abd-B is increased in the presence of exd.

Abd-B still interacts with hth in the absence of exd.

Interaction in vitro; bait and prey produced as recombinant fusion proteins in baculorvirus and insect cell system.

Interaction in vitro; proteins produced by in vitro translation.

Abd-B, hth and exd form a trimeric complex on [32]P labeled DNA. HX-like motif and C-terminal mutations in Abd-B affect binding to CycE DNA but not to Dll DNA.