FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
RecQ5, spn-A
FlyBase ID
FBig0000098124
Interaction Network
Interactions Browser links
RecQ5 network
spn-A network
Reported Interactions
FBrf0219869-1.Y2H
Reference
Maruyama et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
RecQ5 

RecQ5

bait

fused to GAL4 DNA-binding domain

RecQ5 
spn-A 

Rad51

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
RecQ5 
C-terminal region
sufficient binding region
aa 464-1058
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Maruyama et al., 2012)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Maruyama et al., 2012)
FBrf0219869-2.PD.A
Reference
Maruyama et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
RecQ5 

RecQ5

bait

GST tag

RecQ5 
spn-A 

Rad51

prey

[35]S label

spn-A 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
RecQ5 
C-terminal region
sufficient binding region
aa 464-1058
spn-A 
central region
sufficient binding region
aa 82-206
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Maruyama et al., 2012)
FBrf0219869-3.coIP.WB
Reference
Maruyama et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
RecQ5 

RecQ5

bait

HA tag

spn-A 

Rad51

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Maruyama et al., 2012)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)