FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
sc, emc
FlyBase ID
FBig0000098156
Interaction Network
Interactions Browser links
sc network
emc network
Reported Interactions
FBrf0075104-2.coIP.A
Reference
Cabrera et al., 1994
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sc 

T4

bait

[35]S label

emc 

EMC

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced and labeled by in vitro translation; prey produced and labeled by in vitro translation.

(Cabrera et al., 1994)

The emc-sc interaction is stronger than the emc-ac interaction, which is stronger than the emc-l(1)sc interaction.

(Cabrera et al., 1994)

emc blocks the DNA-binding activity of sc-da heterodimers.

(Cabrera et al., 1994)
FBrf0099085-3.Y2H
Reference
Alifragis et al., 1997
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
emc 

Emc

bait

fused to LexA DNA-binding domain

sc 

Sc

prey

fused to VP16 activation domain

sc 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
sc 
bHLH domain
sufficient binding region
aa 1-178
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/VP16-AD

(Alifragis et al., 1997)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Alifragis et al., 1997)

emc interacts with sc and da, and more weakly with ac, but not with ase.

(Alifragis et al., 1997)
FBrf0102176-8.Y2H
Reference
Poortinga et al., 1998
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
emc 

Emc

bait

fused to LexA DNA-binding domain

sc 

Scute

prey

fused to VP16 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Poortinga et al., 1998)

Two-hybrid system: yeast LexA-BD/VP16-AD

(Poortinga et al., 1998)
FBrf0241977-235.Y2H
Reference
Shokri et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
emc 

emc

bait

fused to GAL4 DNA-binding domain

sc 

sc

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Shokri et al., 2019)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Shokri et al., 2019)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (4)