FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Nf1, Fak
FlyBase ID
FBig0000098161
Interaction Network
Interactions Browser links
Nf1 network
Fak network
Reported Interactions
FBrf0220000-1.coIP.WB
Reference
Tsai et al., 2012
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 1embryonic stage 17blastoderm stagegastrula stageembryonic stage 8dorsal closure stageembryonic stage 9embryonic stage 6early extended germ band stageembryonic cycle 3extended germ band stageembryonic stage 4embryonic cycle 9embryonic stage 2late extended germ band stageembryonic stage 5embryonic stage 11embryonic stage 3embryonic stage 12embryonic stage 15embryonic cycle 6embryonic cycle 5embryonic cycle 8embryonic cycle 7embryonic stage 13embryonic cycle 4embryonic stage 14embryonic stage 10embryonic stage 7embryonic stage 16
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Nf1 

NF1

bait
Fak 

Fak56

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Tsai et al., 2012)
FBrf0220000-2.coIP.WB
Reference
Tsai et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Fak 

Fak56

bait

Myc tag

Nf1 

NF1

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Tsai et al., 2012)

Binding of Nf1 does not require phosphorylation of Fak, but is inhibited by exposure to ionomycin (which increase Ca[2+] influx).

(Tsai et al., 2012)

Interaction tested in the presence of calk intestinal phosphatase, or using a Fak Y430F mutation, both of which abolish Fak phosphorylation.

(Tsai et al., 2012)
FBrf0220000-3.coIP.WB
Reference
Tsai et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Nf1 

NF1

bait

FLAG tag

Fak 

Fak56

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Tsai et al., 2012)

Binding of Nf1 does not require phosphorylation of Fak, as mutations that abolish Fak phosphorylation (Y430F) do not affect the interaction.

(Tsai et al., 2012)
FBrf0220000-4.PD.WB
Reference
Tsai et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Nf1 

NF1

bait

GST tag

Nf1 
Fak 

Fak56

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Nf1 
N-terminal region
sufficient binding region
aa 1-408
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from S2 cell extract.

(Tsai et al., 2012)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)