FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
smo, Pp4-19C
FlyBase ID
FBig0000098195
Interaction Network
Interactions Browser links
smo network
Pp4-19C network
Reported Interactions
FBrf0206597-1.coIP.WB
Reference
Jia et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
smo 

Smo

bait

Myc tag

smo 
smo 
Pp4-19C 

PP4

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
smo 
C-terminal region
sufficient binding region
aa 556-1035
smo 
C-terminal region
necessary binding region
aa 626-678
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Jia et al., 2009)
FBrf0206597-2.coIP.WB
Reference
Jia et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
smo 

Smo

bait

FLAG tag

smo 
smo 
Pp4-19C 

PP4

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
smo 
C-terminal region
sufficient binding region
aa 556-678
smo 
C-terminal region
necessary binding region
aa 626-678
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Jia et al., 2009)
FBrf0206597-4.PD.WB
Reference
Jia et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
smo 

Smo

bait

GST tag

smo 
Pp4-19C 

PP4

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
smo 
C-terminal region
sufficient binding region
aa 557-686
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transfected S2 cell extract.

(Jia et al., 2009)

Pre-treatment of S2 cells with hh-containing conditioned medium reduces the interaction of Pp4-19C in cell extracts with GST-smo.

(Jia et al., 2009)

The interaction of smo with Pp4-19C is attenuated by cos RNAi.

(Jia et al., 2009)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)