FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Skp2, dap
FlyBase ID
FBig0000098250
Interaction Network
Interactions Browser links
Skp2 network
dap network
Reported Interactions
FBrf0221694-1.coIP.WB
Reference
Dui et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Skp2 

dSkp2

bait

FLAG tag

dap 

Dap

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The interaction of Skp2 with dap is enhanced by the presence of Cks85A.

(Dui et al., 2013)

Coexpression of Skp2 increases dap polyubiquitination and reduces the level of dap protein, while RNAi against Skp2 increases the level of dap protein.

(Dui et al., 2013)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Dui et al., 2013)
FBrf0221694-4.coIP.WB
Reference
Dui et al., 2013
Description
physical association
Assay
Collection
Source/Stage
eye / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
dap 

Dap

bait

Myc tag

Skp2 

dSkp2

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Coexpression of Skp2 reduces the level of dap protein, while RNAi against Skp2 increases the level of dap protein.

(Dui et al., 2013)

Source was adult head of transgenic fly line; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.

(Dui et al., 2013)

Transgenic constructs expressed in the eye using the GMR-GAL4 driver.

(Dui et al., 2013)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)