FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
smg, cup
FlyBase ID
FBig0000098252
Interaction Network
Interactions Browser links
smg network
cup network
Reported Interactions
FBrf0167556-1.PD.MS
Reference
Nelson et al., 2004
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 1embryonic stage 6blastoderm stageembryonic stage 5embryonic stage 3embryonic cycle 6embryonic cycle 8embryonic cycle 5embryonic cycle 7embryonic cycle 4embryonic cycle 3embryonic stage 4embryonic stage 2embryonic cycle 9
Cell line used
Participants
Corresponds to
Reported as
Role
Note
smg 

Smg

bait

GST fusion

smg 
cup 

Cup

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
smg 
region
sufficient binding region
aa 583-763
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro using embryonic extract; bait produced as a recombinant fusion protein; prey present in extract (produced from endogenous gene).

(Nelson et al., 2004)
FBrf0167556-10.coIP.WB
Reference
Nelson et al., 2004
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 1embryonic stage 6blastoderm stageembryonic stage 5embryonic stage 3embryonic cycle 6embryonic cycle 8embryonic cycle 5embryonic cycle 7embryonic cycle 4embryonic cycle 3embryonic stage 4embryonic stage 2embryonic cycle 9
Cell line used
Participants
Corresponds to
Reported as
Role
Note
smg 

Smg

bait
cup 

Cup

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Nelson et al., 2004)

In the presence of RNase during immunoprecipitation this interaction was still observed.

(Nelson et al., 2004)
FBrf0167556-2.PD.A
Reference
Nelson et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
smg 

Smg

bait

GST fusion

smg 
cup 

Cup

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
smg 
region
sufficient binding region
aa 583-763
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein; prey produced and labeled by in vitro translation.

(Nelson et al., 2004)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)