FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
ci, Kap-α3
FlyBase ID
FBig0000098266
Interaction Network
Interactions Browser links
ci network
Kap-α3 network
Reported Interactions
FBrf0212658-2.Y2H
Reference
Seong et al., 2010
Description
physical association
Assay
Collection
Source/Stage
organism / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ci 

Ci

bait

fused to LexA DNA-binding domain

ci 
Kap-α3 

importin α3

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ci 
N-terminal region
sufficient binding region
aa 1-441
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast LexA-BD/GAL4-AD

(Seong et al., 2010)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from adult cDNA expression library).

(Seong et al., 2010)
FBrf0212658-3.coIP.WB
Reference
Seong et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
CME-W1-Cl.8+
Participants
Corresponds to
Reported as
Role
Note
ci 

Ci

bait

HA tag

Kap-α3 

Importin α3

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

rdx and Kap-α3 compete for binding to ci.

(Seong et al., 2010)

Source was cell extract of clone8 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Seong et al., 2010)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)