FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
pAbp, gw
FlyBase ID
FBig0000098301
Interaction Network
Interactions Browser links
pAbp network
gw network
Reported Interactions
FBrf0212673-1.coIP.WB
Reference
Huntzinger et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
gw 

GW182

bait

GFP tag

gw 
gw 
gw 
pAbp 

DmPABPC1

prey

V5 tag

pAbp 
pAbp 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
pAbp 
RRM1-4 domain
sufficient binding region
unspecified
pAbp 
RRM1 domain
mutation decreasing interaction
unspecified

deletion of RRM1 domain

gw 
silencing domain
necessary binding region
unspecified
gw 
silencing domain
sufficient binding region
unspecified
gw 
silencing domain
mutation increasing interaction
958,959962

V958P,Q959P, coordinates relative to gw-PA

E962Q, coordinates relative to gw-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Huntzinger et al., 2010)
FBrf0212673-3.PD.MW
Reference
Huntzinger et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
gw 

GW182

bait

GST tag

gw 
gw 
pAbp 

DmPABPC1

prey

MBP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
gw 
silencing domain
sufficient binding region
unspecified
gw 
PAM2 domain
necessary binding region
unspecified
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Huntzinger et al., 2010)
FBrf0215100-3.coIP.WB
Reference
Zekri et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
gw 

GW182

bait

GFP tag

gw 
gw 
pAbp 

PABPC1

prey

V5 tag

pAbp 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
gw 
silencing domain
necessary binding region
aa 861-1384
gw 
silencing domain
sufficient binding region
aa 861-1384
pAbp 
N-terminal RRM domains
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Zekri et al., 2009)

gw interacts with pAbp, but not with components of the deadenylase or decapping complexes (Pop2, Dcp1, eIF-4E or eIF4G).

(Zekri et al., 2009)
FBrf0215100-7.PD.WB
Reference
Zekri et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
pAbp 

PABPC1

bait

GFP tag

gw 

GW182

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

pAbp-eIF4G complexes were immunoprecipitated from S2 cell extracts, then incubated with separate S2 cell extracts containing increasing amounts of gw protein.

(Zekri et al., 2009)

gw competes with eIF4G for binding to pAbp. Increasing amounts of gw displace eIF4G bound to pAbp.

(Zekri et al., 2009)

Interaction in vitro; bait derived from transfected S2 cell extract; prey derived from transfected S2 cell extract.

(Zekri et al., 2009)
FBrf0216898-1.coIP.WB
Reference
Fukaya and Tomari, 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
gw 

GW182

bait

HA tag

pAbp 

PABP

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Addition of Paip2 reduces gw-pAbp interaction.

(Fukaya and Tomari, 2011)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Fukaya and Tomari, 2011)
FBrf0220586-4.coIP.WB
Reference
Huntzinger et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
gw 

GW182

bait

GFP tag

gw 
gw 
gw 
gw 
pAbp 

PABP

prey

V5 tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
gw 
silencing domain
sufficient binding region
aa 825-1384
gw 
M2 region
mutation decreasing interaction

six Trp residues mutated to Ala (\'6xW\' mutant)

gw 
PAM2 motif
mutation decreasing interaction
aa 961

F961A

gw 
CIM-1 motif
mutation decreasing interaction
aa 870-875

S870A, R871A, W875A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Huntzinger et al., 2013)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (4)