FB2026_03 , released September 17, 2026
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
Tfb5, CG31917
FlyBase ID
FBig0000098302
Interaction Network
Interactions Browser links
Tfb5 network
CG31917 network
Reported Interactions
FBrf0219503-3.Y2H
Reference
Herrera-Cruz et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Tfb5 

Dmp8

bait

fused to GAL4 DNA-binding domain

CG31917 

Dmp18

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Herrera-Cruz et al., 2012)

This interaction was not observed in the reciprocal experiment (where bait and prey roles were switched).

(Herrera-Cruz et al., 2012)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Herrera-Cruz et al., 2012)
FBrf0219503-8.PD.A
Reference
Herrera-Cruz et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
CG31917 

Dmp18

bait

GST tag

CG31917 
Tfb5 

Dmp8

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
CG31917 
C-terminal region
sufficient binding region
aa 49-152

weak binding

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Herrera-Cruz et al., 2012)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)