FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Gene: Dmel\Tfb5
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General Information
Symbol
Dmel\Tfb5
Species
D. melanogaster
Name
Transcription factor B5
Annotation Symbol
CG42373
Feature Type
protein_coding_gene
FlyBase ID
FBgn0265968
Gene Model Status
Current
Stock Availability
7 publicly available
Gene Summary
Transcription factor B5 (Tfb5) encodes a subunit of the basal transcription and DNA repair factor TFIIH, which operates in transcription and DNA repair. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)
All Summaries
Also Known As

p8, TTDA, Dmp8, lincRNA.843

Key Links
Genomic Location
Cytogenetic map
25C1-25C1
Sequence location
Recombination map
2-15
RefSeq locus
NT_033779 REGION:5009931..5010834
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (8 terms)
Molecular Function (0 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (0 terms)
Biological Process (5 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
involved_in nucleotide-excision repair
inferred from direct assay
(Aguilar-Fuentes et al., 2008)
involved_in response to UV
inferred from direct assay
(Aguilar-Fuentes et al., 2008)
involved_in transcription by RNA polymerase II
inferred from direct assay
(Aguilar-Fuentes et al., 2008)
Terms Based on Predictions or Assertions (4 terms)
CV Term
Evidence
References
involved_in nucleotide-excision repair, preincision complex assembly
inferred from biological aspect of ancestor with PANTHER:PTN002005445
(GO Reference Genome Project, 2011-)
involved_in nucleotide-excision repair
inferred from electronic annotation with InterPro:IPR009400
(InterPro Project Members, 2004-)
involved_in transcription by RNA polymerase II
inferred from biological aspect of ancestor with PANTHER:PTN002005445
(GO Reference Genome Project, 2011-)
involved_in transcription initiation at RNA polymerase II promoter
inferred from electronic annotation with InterPro:IPR009400
(InterPro Project Members, 2004-)
Cellular Component (3 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
located_in nucleus
inferred from direct assay
(Aguilar-Fuentes et al., 2008)
part_of transcription factor TFIIH core complex
inferred from direct assay
(Aguilar-Fuentes et al., 2008)
part_of transcription factor TFIIH holo complex
inferred from direct assay
(Aguilar-Fuentes et al., 2008)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
part_of transcription factor TFIIH core complex
inferred from electronic annotation with InterPro:IPR009400
(InterPro Project Members, 2004-)
inferred from biological aspect of ancestor with PANTHER:PTN002005445
(GO Reference Genome Project, 2011-)
part_of transcription factor TFIIH holo complex
inferred from biological aspect of ancestor with PANTHER:PTN002005445
(GO Reference Genome Project, 2011-)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Summaries
Gene Snapshot
Transcription factor B5 (Tfb5) encodes a subunit of the basal transcription and DNA repair factor TFIIH, which operates in transcription and DNA repair. [Date last reviewed: 2019-03-14]
Gene Group (FlyBase)
TRANSCRIPTION FACTOR II H - CORE COMPLEX -
The general transcription factor complex Transcription factor II H (TFIIH) core complex, is a subcomplex of the TFIIH holoenzyme required in RNA pol II transcription with an ATP-dependent DNA helicase activity required for promoter DNA opening. TFIIH also functions in nucleotide excision repair (NER). After complex loading and the verification step of NER, the cyclin-dependent protein kinase-activating kinase (CAK) subcomplex dissociates and the TFIIH core complex functions independently. (Adapted from PMID:25693126, PMID:21592869 and PMID:30798933).
Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\Tfb5 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry B7Z018)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Gene model reviewed during 5.45

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Tfb5-RB
FBtr0301130
NM_001144319
846
73
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
Tfb5-PB
FBpp0290353
8.4
73
4.54
B7Z018
NP_001137791
ACL82997
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Tfb5 using the Feature Mapper tool.

External Data
Crossreferences
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

4.38

Transcript Expression
Polypeptide Expression
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Tfb5 in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 3 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 8 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Tfb5
Transgenic constructs containing regulatory region of Tfb5
Aberrations (Deficiencies and Duplications) ( 3 )
Inferred from experimentation ( 3 )
Gene disrupted in
Df(2L)BSC172
(Herrera-Cruz et al., 2012)
Df(2L)Exel7022
(Herrera-Cruz et al., 2012)
Gene not disrupted in
Df(2L)BSC110
(Herrera-Cruz et al., 2012)
Inferred from location ( 5 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (1)
Hsap\GTF2H5
13 of 14
Yes
Yes
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (2)
Rnor\Gtf2h5
13 of 14
Yes
Yes
Rnor\LOC120103497
9 of 14
No
Yes
Mus musculus (laboratory mouse) (1)
Mmus\Gtf2h5
13 of 14
Yes
Yes
Xenopus tropicalis (Western clawed frog) (1)
Xtro\gtf2h5
6 of 13
Yes
Yes
Danio rerio (Zebrafish) (1)
Drer\gtf2h5
13 of 14
Yes
Yes
Caenorhabditis elegans (Nematode, roundworm) (1)
Cele\gtf-2H5
12 of 14
Yes
Yes
Anopheles gambiae (African malaria mosquito) (0)
Arabidopsis thaliana (thale-cress) (2)
Atha\AT1G12400
8 of 13
Yes
Yes
Atha\AT1G62886
7 of 13
No
Yes
Saccharomyces cerevisiae (Brewer's yeast) (1)
Scer\TFB5
9 of 13
Yes
Yes
Schizosaccharomyces pombe (Fission yeast) (1)
Spom\tfb5
9 of 12
Yes
Yes
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:Tfb5. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 1 )
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    GTF2H5; general transcription factor IIH subunit 5
    13 of 14
    608780
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    Summary of Genetic Interactions
    Interaction Browsers
    View in FlyBase Interactions Browser   View in MIST tool (external link)
    Please look at the allele data for full details of the genetic interactions
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    hay
    suppressible
    Tfb5
    (Aguilar-Fuentes et al., 2008)
    mrn
    suppressible
    Tfb5
    (Aguilar-Fuentes et al., 2008)
    External Data
    Linkouts
    DroID - A comprehensive database of gene and protein interactions.
    MIST (genetic) - An integrated Molecular Interaction Database
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Pathways
    Class of Gene
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    2L
    Recombination map
    2-15
    Cytogenetic map
    25C1-25C1
    Sequence location
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    25C1-25C1
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    25B10-25B10
    (Oh et al., 2003)
    Experimentally Determined Recombination Data
    Location

    2-15

    (FlyBase, 2016)

    2-16.5

    (Comeron et al., 2012)
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (7)
    Genomic Clones (11)
     

    Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

    cDNA Clones (7)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequenced
    BDGP DGC clones
    Other clones
    Drosophila Genomics Resource Center cDNA clones

    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

    cDNA Clones, End Sequenced (ESTs)
    BDGP DGC clones
    Other clones
    RNAi and Array Information
    Linkouts
    Antibody Information
    Laboratory Generated Antibodies
     

    polyclonal

    (Herrera-Cruz et al., 2012)
    Commercially Available Antibodies
     
    Cell Line Information
    Publicly Available Cell Lines
     
      Other Stable Cell Lines
       
        Other Comments

        Sequence comparison across multiple species (MSA) available in Supplementary Information.

        (Takahashi et al., 2020)

        1 allele of l(2)SH1279 recovered in a P-insertion screen.

        (Oh et al., 2003)
        Relationship to Other Genes
        Source for database merge of

        Source for merge of: CG14037 CG14038

        (FlyBase Genome Annotators, 2002-2003)

        Source for merge of: Tfb5 l(2)SH1279

        (Herrera-Cruz et al., 2012)
        Additional comments

        One or more of the processed transcripts for this gene contain(s) two non-overlapping open reading frames (ORFs). The non-overlapping ORFs are represented by CG31917 and CG42373. The ORF of release 5.10 annotation CG42373 is identical to the ORF of release 2 annotation CG14037 and the ORF of release 5.10 annotation CG31917 is identical to the ORF of release 2 annotation CG14038.

        (FlyBase Genome Annotators, 2008)

        Annotations CG14037 and CG14038 merged as CG31917 in release 3 of the genome annotation.

        (FlyBase Genome Annotators, 2002-2003)
        Nomenclature History
        Source for database identify of

        Source for identity of: Tfb5 CG42373

        (Hayden and Bosco, 2008)
        Nomenclature comments
        Etymology

        Named "Tfb5" after the S. cerevisiae ortholog.

        (FlyBase, 2009-)
        Synonyms and Secondary IDs (16)
        Datasets (0)
        Study focus (0)
        Experimental Role
        Project
        Project Type
        Title
        Study result (0)
        Result
        Result Type
        Title
        External Crossreferences and Linkouts ( 40 )
        Sequence Crossreferences
        NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
        GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
        GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
        RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
        UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
        UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
        Other crossreferences
        AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
        BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
        DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
        EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
        FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
        InterPro - A database of protein families, domains and functional sites
        KEGG Genes - Molecular building blocks of life in the genomic space.
        MARRVEL_MODEL - MARRVEL (model organism gene)
        Linkouts
        Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
        DroID - A comprehensive database of gene and protein interactions.
        FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
        KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
        MIST (genetic) - An integrated Molecular Interaction Database
        MIST (protein-protein) - An integrated Molecular Interaction Database
        Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
        References (36)