Interaction in vitro; protein derived from conditioned medium of transfected HEK293 cells.

Lac-Fc fusion protein was recovered from conditioned medium and coupled to anti-Fc-coated fluorescent beads. Homophilic Lac interaction was inferred from bead aggregation under fluorescence microscopy.

Source was live S2 cells; proteins produced from transfected constructs.

Cells expressing Gli fail to form aggregates when combined with Lac expressing cells, indicating that Lac and Gli do not interact in this assay.

S2 cells were transfected with GFP and Lac expression vectors and aggregation was observed with transmission and confocal microscopy.