FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Rho1, capu
FlyBase ID
FBig0000098408
Interaction Network
Interactions Browser links
Rho1 network
capu network
Reported Interactions
FBrf0111959-1.PD.A
Reference
Magie et al., 1999
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rho1 

Rho1

bait

GST tag

capu 

capu

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Magie et al., 1999)

capu binds GTP bound Rho1.

(Magie et al., 1999)
FBrf0191267-1.PD.A
Reference
Rosales-Nieves et al., 2006
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rho1 

Rho

bait

GST tag

Rho1 
Rho1 
capu 

Capu

prey

[35]S label

capu 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Rho1 
region
mutation increasing interaction
aa 14

G14V, mimics GTP-bound state

Rho1 
region
mutation decreasing interaction
aa 19

T19N, mimics GDP-bound state

capu 
Rho-binding domain
sufficient binding region
aa 125-250

\'N3\'

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Rosales-Nieves et al., 2006)
FBrf0191267-3.coIP.WB
Reference
Rosales-Nieves et al., 2006
Description
physical association
Assay
Collection
Source/Stage
ovary / adult stagefemale
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rho1 

Rho

bait
capu 

Capu

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult female ovary of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Rosales-Nieves et al., 2006)
FBrf0208460-8.coIP.WB
Reference
Liu et al., 2009
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rho1 

Rho1

bait
capu 

Capu

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Liu et al., 2009)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)