FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Synj, Dap160
FlyBase ID
FBig0000098419
Interaction Network
Interactions Browser links
Synj network
Dap160 network
Reported Interactions
FBrf0103356-5.FW.MW
Reference
Roos and Kelly, 1998
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Dap160 

Dap160

bait

GST tag

Dap160 
Synj 

Synaptojanin

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Dap160 
SH3 BCD domains
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type adult head extract.

(Roos and Kelly, 1998)

Prey identification based on comigration of far western band with band detected by immunoblot using anti-synj antisera.

(Roos and Kelly, 1998)
FBrf0167964-2.PD.WB
Reference
Verstreken et al., 2003
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Synj 

Synj

bait

GST tag

Synj 
Dap160 

Dap160

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Synj 
proline-rich domain
sufficient binding region
aa 1-301
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from adult head extract.

(Verstreken et al., 2003)

The proline-rich domain of synj interacts with endoA and Dap160, but not to shi or n-syb.

(Verstreken et al., 2003)
FBrf0167964-3.PD.WB
Reference
Verstreken et al., 2003
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Dap160 

Dap160

bait

GST tag

Dap160 
Synj 

Synj

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Dap160 
SH3 C and D domains
sufficient binding region

weak binding to the SH3 \'C\' domain in isolation

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from adult head extract.

(Verstreken et al., 2003)
FBrf0225491-3.coIP.WB
Reference
Chen et al., 2014
Description
physical association
Assay
Collection
Source/Stage
adult head / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Synj 

Synj

bait
Dap160 

DAP160

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The Synj-Dap160 interaction is reduced in a mnb mutant background.

(Chen et al., 2014)

Source was adult heads of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Chen et al., 2014)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)